GB/T 44367-2024 化妆品中限用组分二氨基嘧啶氧化物的测定 高效液相色谱法

ICS

71.100.70

CCS

Y42

中华人民共和国国家标准

GB/T44367—2024

化妆品中限用组分二氨基嘧啶氧化物的

测定高效液相色谱法

Determinationofrestricted2,4-diamino-pyrimidine-3-oxideincosmetics—

Highperformanceliquidchromatography

2024-08-23发布2025-03-01实施

国家市场监督管理总局发布

国家标准化管理委员会

GB/T44367—2024

目次

前言

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Ⅲ

1

范围

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2

规范性引用文件

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3

术语和定义

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4

原理

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5

试剂和材料

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6

仪器设备

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试样制备和试验步骤

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7.1

试样制备

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7.2

高效液相色谱测定参考条件

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7.3

标准工作曲线绘制

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7.4

试样测定

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7.5

空白试验

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试验数据处理

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9

回收率

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10

精密度

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附录A（资料性）

二氨基嘧啶氧化物标准品的信息

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附录B（资料性）

二氨基嘧啶氧化物标准溶液的色谱图和光谱图

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Ⅰ

GB/T44367—2024

前言

本文件按照GB/T1.1—2020《标准化工作导则第1部分：标准化文件的结构和起草规则》的规

定起草。

请注意本文件的某些内容可能涉及专利。本文件的发布机构不承担识别专利的责任。

本文件由中国轻工业联合会提出。

本文件由全国香料香精化妆品标准化技术委员会（SAC/TC257）归口。

本文件起草单位：苏州世谱检测技术有限公司、苏科世谱生物技术（苏州）有限公司、苏州质量检

测科学研究院、苏州市科耐瑞检测技术有限公司、江苏奇力康皮肤药业有限公司、广州质量监督检测研

究院、上海香料研究所有限公司、江苏省产品质量监督检验研究院、完美（广东）日用品有限公司、河

北省食品检验研究院、江苏省食品药品监督检验研究院、清华大学、常州市食品药品纤维质量监督检验

中心、深圳市药品检验研究院、苏州市药品检验检测研究中心、苏州大学、常州大学、广东悠质检测技

术有限公司、珀莱雅化妆品股份有限公司、美出莱（杭州）化妆品有限责任公司、浙江方圆检测集团股

份有限公司、上海永熙信息科技有限公司、上海其然生物科技有限公司。

本文件主要起草人：何霜、卢剑、朱丹丹、方方、李建祥、张岩、吴克、代丹、郭燕华、结莉、

康薇、王怡、张迎阳、叶嘉荣、叶竹洪、杨斌、刘丹阳、寻知庆、宋淑文、汪晨霞、黄宏霞、李适炜、

孙静霞、萧慧珍、杨铭、孙淑蓉、喻竟、袁旻嘉、卢应红、姜雷、高龙、顾盼、刘群林、高红影、

朱钇蓉。

Ⅲ

GB/T44367—2024

化妆品中限用组分二氨基嘧啶氧化物的

测定高效液相色谱法

警示─使用本文件的人员应有正规实验室工作的实践经验。本文件并未指出所有可能的安全问题。

使用者有责任采取适当的安全和健康措施，并保证符合国家有关规定的条件。

1范围

本文件描述了化妆品中二氨基嘧啶氧化物的高效液相色谱测定方法的原理、试剂和材料、仪器设

备、试样制备和试验步骤、试验数据处理、回收率和精密度等内容。

本文件适用于水剂类、乳液类、膏霜类等发用化妆品中二氨基嘧啶氧化物的测定。

本文件方法检出限为3.0mg/kg，定量限为10.0mg/kg。

2规范性引用文件

下列文件中的内容通过文中的规范性引用而构成本文件必不可少的条款。其中，注日期的引用文

件，仅该日期对应的版本适用于本文件；不注日期的引用文件，其最新版本（包括所有的修改单）适用

于本文件。

GB/T6682分析实验室用水规格和试验方法

3术语和定义

本文件没有需要界定的术语和定义。

4原理

试样经10%甲醇涡旋振荡，超声提取，离心，正己烷液液萃取净化过滤后，得到目标物待测溶液。

将目标物待测溶液注入高效液相色谱仪，采用二极管阵列检测器检测，保留时间和紫外吸收光谱图定

性，外标法定量。

5试剂和材料

除非另有规定，仅使用色谱纯试剂。

5.1水：GB/T6682，一级。

5.2甲醇。

5.3正己烷。

5.410%（体积分数）甲醇溶液：取10mL甲醇（5.2）加90mL水（5.1），混合均匀。

5.5二氨基嘧啶氧化物标准品：纯度不小于98%，标准品的英文名称、CAS号、分子式、相对分子质

量和化学结构式见附录A中表A.1。

5.6标准储备液：准确称取标准品（5.5）0.01g（精确至0.0001g）于10mL容量瓶中，用甲醇

（5.2）溶解并稀释至刻度，即得质量浓度为1000µg/mL的标准储备液。贮存期为：2℃~8℃避光保

存，6个月。

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