GB/T 44350-2024 油菜蜂蜜中丁香酸甲酯的测定 高效液相色谱法

ICS

67.180.10

CCS

B47

中华人民共和国国家标准

GB/T44350—2024

油菜蜂蜜中丁香酸甲酯的测定

高效液相色谱法

Determinationofmethylsyringateinrapehoney—Highperformanceliquid

chromatographymethod

2024-09-29发布2025-04-01实施

国家市场监督管理总局

发布

国家标准化管理委员会

GB/T44350—2024

目次

前言

Ⅲ

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1

范围

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2

规范性引用文件

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3

术语和定义

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4

原理

1

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5

试剂和材料

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6

仪器设备

2

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7

分析步骤

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7.1

试样制备

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7.2

空白试验

2

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7.3

平行试验

2

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7.4

测定

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结果计算

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9

定量限

3

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10

精密度

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附录A（资料性）

丁香酸甲酯标准工作液的高效液相色谱图

4

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Ⅰ

GB/T44350—2024

前言

本文件按照GB/T1.1—2020《标准化工作导则第1部分：标准化文件的结构和起草规则》的规

定起草。

请注意本文件的某些内容可能涉及专利。本文件的发布机构不承担识别专利的责任。

本文件由中华全国供销合作总社提出。

本文件由全国蜂产品标准化技术委员会（SAC/TC601）归口。

本文件起草单位：中国计量大学、安徽蜜之源食品集团有限公司、广州金和技术检测有限公司、中

科标准（宁德）科技有限公司、深圳市威标检测技术有限公司、南京海关动植物与食品检测中心、金华

市农业科学研究院、中国农业科学院蜜蜂研究所、浙江省乡村振兴促进中心、绿城农科检测技术有限公

司、浙江华才检测技术有限公司、浙江省畜牧技术推广与种畜禽监测总站、江苏国测检测技术有限公

司、深圳昱朋科技有限公司、齐齐哈尔市检验检测中心、青岛中一监测有限公司、湖州市蜂业研究院。

本文件主要起草人：李红亮、朱鑫麗、林宏、苏晓玲、吴帆、乔江涛、黄金晶、王志军、林钗、

何丽、李加芬、汪腊云、李奎、林影、黄泳铭、王韦岗、娄喜山、钟巧霞、邱凯。

Ⅲ

GB/T44350—2024

油菜蜂蜜中丁香酸甲酯的测定

高效液相色谱法

1范围

本文件描述了测定油菜蜂蜜中丁香酸甲酯含量的高效液相色谱方法。

本文件适用于油菜蜂蜜中丁香酸甲酯含量的测定。

2规范性引用文件

下列文件中的内容通过文中的规范性引用而构成本文件必不可少的条款。其中，注日期的引用文

件，仅该日期对应的版本适用于本文件；不注日期的引用文件，其最新版本（包括所有的修改单）适用

于本文件。

GB/T6682分析实验室用水规格和试验方法

3术语和定义

本文件没有需要界定的术语和定义。

4原理

油菜蜂蜜样品中的丁香酸甲酯经弱阴离子交换固相萃取柱净化和富集后，经C18高效液相色谱柱分

离，紫外或二极管阵列检测器检测（最大吸收波长为276nm），外标峰面积法定量。

5试剂和材料

除非另有说明，所有试剂均为分析纯，水为GB/T6682规定的一级水。

5.1乙腈（色谱纯）。

5.2氨水：浓度为25%～28%（质量分数）。

5.3丁香酸甲酯（CHO）标准品：CAS号884﹘35﹘5，纯度不小于98%，或采用标准物质/标准样品。

10125

5.4丁香酸甲酯标准储备液（1000mg/L）：准确称取0.1000g±0.0001g丁香酸甲酯标准品（5.3）于

25mL烧杯中，用乙腈（5.1）溶解后，转移至100mL容量瓶中，定容至刻度，摇匀，得1000mg/L的

丁香酸甲酯标准储备液。置于4℃避光保存，有效期6个月。

5.5丁香酸甲酯标准中间液（100mg/L）：准确吸取1000mg/L的丁香酸甲酯标准储备液（5.4）1.0mL

于10mL容量瓶中，用水稀释并定容至刻度，摇匀，得100mg/L的丁香酸甲酯标准中间液。置于4℃

避光保存，有效期3个月。

5.6丁香酸甲酯标准工作液：分别准确吸取0.1mL、0.2mL、0.4mL、1.0mL、4.0mL、10mL丁香

酸甲酯标准中间液（5.5）至10mL容量瓶中，分别加水稀释并定容至刻度，摇匀，得到系列质量浓度为

1mg/L、2mg/L、4mg/L、10mg/L、20mg/L、40mg/L，现配现用。以上溶液与100mg/L的丁香

酸甲酯标准中间液（5.5）共同组成丁香酸甲酯标准工作液，用于标准曲线制作（7.4.2）。

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