GB/T 44396-2024 化学品 稀有鮈鲫胚胎急性毒性试验

ICS13.300

CCSA80

中华人民共和国国家标准

GB/T44396—2024

化学品稀有鮈鲫胚胎急性毒性试验

Chemicals—Rareminnow（Gobiocyprisrarus）embryoacutetoxicitytest

2024⁃08⁃23发布2025⁃03⁃01实施

国家市场监督管理总局

发布

国家标准化管理委员会

GB/T44396—2024

目次

前言··························································································································Ⅲ

1范围·······················································································································1

2规范性引用文件········································································································1

3术语和定义··············································································································1

4受试物信息··············································································································1

5试验原理·················································································································2

6设备与材料··············································································································2

7试验前期准备···········································································································3

8试验程序·················································································································4

9质量控制·················································································································6

10数据与报告············································································································7

附录A（资料性）稀有鮈鲫胚胎的正常发育图····································································8

附录B（资料性）试验用水水质条件·················································································9

附录C（资料性）稀有鮈鲫胚胎急性毒性试验鱼卵鉴别参考图··············································10

附录D（资料性）24孔细胞培养板布局···········································································12

参考文献····················································································································13

Ⅰ

GB/T44396—2024

前言

本文件按照GB/T1.1—2020《标准化工作导则第1部分：标准化文件的结构和起草规则》的规

定起草。

请注意本文件的某些内容可能涉及专利。本文件的发布机构不承担识别专利的责任。

本文件由全国危险化学品管理标准化技术委员会（SAC/TC251）提出并归口。

本文件起草单位：上海市检测中心、生态环境部固体废物与化学品管理技术中心、中国科学院水生

生物研究所、沈化测试技术（南通）有限公司、宁波海关技术中心、苏州华测安评技术服务有限公司、

贵州健安德科技有限公司、广东省科学院微生物研究所（广东省微生物分析检测中心）。

本文件主要起草人：陈晓倩、杨婧、张瑛、刘亚楠、殷浩文、刘洪英、刘晓建、王剑伟、何勇凤、李子璇、

刘汉伟、章跃龙、皮天星、田宝莲、杨鸿波、董榕贵、吴欣颖。

Ⅲ

GB/T44396—2024

化学品稀有鮈鲫胚胎急性毒性试验

1范围

本文件描述了化学品对稀有鮈鲫胚胎急性毒性试验方法，包括受试物所需信息、试验原理、仪器设

备、试验前期准备、试验程序、质量控制、数据与报告。

本文件适用于测试化学品对稀有鮈鲫胚胎的急性毒性效应。

2规范性引用文件

下列文件中的内容通过文中的规范性引用而构成本文件必不可少的条款。其中，注日期的引用文

件，仅该日期对应的版本适用于本文件；不注日期的引用文件，其最新版本（包括所有的修改单）适用于

本文件。

GB/T27861化学品鱼类急性毒性试验

3术语和定义

GB/T27861界定的以及下列术语和定义适用于本文件。

3.1

静态试验statictest

以整个试验过程中试验介质不更换、不流动的方式进行的试验。

3.2

半静态试验semi⁃statictest

以定期（如24h）更换试验介质的方式进行的试验。

3.3

流水式试验flow⁃throughtest

以整个试验过程中试验介质自动、持续地被更换的方式进行的试验。

3.4

半数致死浓度medianlethalconcentration

引起50%的受试鱼卵死亡的受试物浓度。

注：以LC50表示。

3.5

板内质控internalplatecontrol

试验过程中，24孔细胞培养板上用于验证细胞培养板生产厂家或操作人员操作导致的潜在污染

风险以及可能影响试验结果的任何孔板效应（如温度梯度）的4个培养孔。

4受试物信息

受试物所需信息包括：

a）CAS号；

b）结构式；

1