DB2305/T 020-2024 牛病毒性腹泻病毒PCR检测规程

DB2305/T 020-2024 PCR detection protocol for bovine viral diarrhea virus

黑龙江省地方标准简体中文现行页数：8页 | 格式：PDF

基本信息

标准号

DB2305/T 020-2024

标准类型

黑龙江省地方标准

标准状态

现行

中国标准分类号（CCS）

B41 动物检疫、兽医与疫病防治

国际标准分类号（ICS）

65.020.30 动物饲养和繁殖

发布日期

2024-08-20

实施日期

2024-09-20

发布单位/组织

双鸭山市市场监督管理局

归口单位

双鸭山市农业农村局

适用范围

本文件适用于牛病毒性腹泻病毒PCR检测。

发布历史

2024年08月

DB2305/T 020-2024　牛病毒性腹泻病毒PCR检测规程

当前标准现行 2024-08-20

文前页预览

在线预览全文

研制信息

起草单位：: 双鸭山市农业技术综合服务中心、双鸭山市基本建设项目服务中心、黑龙江农业职业技术学院。

起草人：: 王宇婷、白鹭、刘云、张红军、杜航、张荣翔、秦藕菊。

出版信息：: 页数：8页 | 字数：- | 开本： -

内容描述

ICS65.020.30

CCSB41

DB2305

双鸭山市地方标准

DB2305/T020—2024

__________________________________________________________________________________________

牛病毒性腹泻病毒PCR检测规程

2024-08-20发布2024-09-20实施

__________________________________________________________________________________________

双鸭山市市场监督管理局发布

DB2305/T020—2024

前言·····························································································································Ⅱ

1范围································································································································1

2规范性引用文件·················································································································1

3术语和定义·······················································································································1

4缩略语·····························································································································1

5主要仪器设备····················································································································1

6试剂································································································································2

7样品的采集与处理··············································································································3

7.1样品采集·······················································································································3

7.2样品处理·······················································································································3

8样品病毒核酸RNA提取········································································································3

9PCR扩增···························································································································3

9.1引物·····························································································································3

9.2反应条件·······················································································································4

10结果判定························································································································4

10.1检测结果成立条件·······························································································

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