DB43/T 2412-2022 鸡冷冻精液制作技术规程

ICS65.020.30

CCSB43

43

湖南省地方标准

DB43/T2412—2022

鸡冷冻精液制作技术规程

Codeofpracticeformakingroosterfrozensemen

2022-07-08发布2022-10-08实施

湖南省市场监督管理局发布

DB43/T2412—2022

目次

前言························································································································Ⅲ

1范围·····················································································································1

2规范性引用文件······································································································1

3术语和定义············································································································1

4准备·····················································································································1

5精液采集与品质检测································································································2

6细管精液制作·········································································································2

7细管精液冷冻·········································································································3

8细管冷冻精液解冻及活力检测····················································································3

9细管冷冻精液保存及解冻应用····················································································4

附录A（资料性）主要器具与耗材················································································5

附录B（规范性）冷冻精液用液配制与保存····································································6

附录C（资料性）精子密度估测方法示例·······································································7

附录D（资料性）精子活力评判方法·············································································8

附录E（资料性）液氮熏蒸法冷冻装置与细管铺放示例·····················································9

附录F（资料性）解冻精液应用及输精效果检测····························································10

参考文献··················································································································11

I

DB43/T2412—2022

II

DB43/T2412—2022

前言

本文件按照GB/T1.1—2020《标准化工作导则第1部分：标准化文件的结构和起草规则》的规

定起草。

请注意本文件的某些内容可能涉及专利。本文件的发布机构不承担识别专利的责任。

本文件由湖南省农业农村厅提出。

本文件由湖南省农业标准化技术委员会归口。

本文件起草单位：湖南省畜牧兽医研究所、全国畜牧总站、浏阳市农业发展事务中心、湖南光大牧

业科技有限公司、浏阳市湘依园生态农业有限公司、湖南陶氏生态禽业科技发展有限公司。

本文件主要起草人：燕海峰、李安定、朱芳贤、宋武、白文娟、李闯、赵晓、张旭、刘伯承、黄璇、

邓萍、伍国强、江为民、冯小花、文平、刘海林、朱斌、陶金彪。

III

DB43/T2412—2022

IV

DB43/T2412—2022

鸡冷冻精液制作技术规程

1范围

本文件规定了鸡冷冻精液制作的准备、精液采集与品质检测、细管精液制作、细管精液冷冻、细管

冷冻精液解冻及活力检测、细管冷冻精液保存及解冻应用。

本文件适用于鸡细管冷冻精液的制作。

2规范性引用文件

下列文件中的内容通过文中的规范性引用而构成本文件必不可少的条款。其中，注日期的引用文件，

仅该日期对应的版本适用于本文件；不注日期的引用文件，其最新版本（包括所有的修改单）适用于本

文件。

GB/T30396牛冷冻精液包装、标签、储存和运输

NY/T1234牛冷冻精液生产技术规程

NY/T3458种鸡人工授精技术规程

NY/T4047家禽精液品质检测方法

3术语和定义

NY/T3458、NY/T4047界定的术语和定义适用于本文件。

4准备

4.1器具与耗材

4.1.1主要器具与耗材准备见附录A。

4.1.2细管标示按NY/T1234的规定执行，其中制作单位名称标示，采用习惯字母、英文或者拼音首

字母。

4.1.3重复使用的器具，使用后立即用清水浸泡。器具清洗和消毒操作按NY/T1234与NY/T3458的

规定执行。

4.1.4细管、细管搓板、细管承载泡沫框等细管精液制作用具使用前置于2℃～5℃保温箱中，温度平

衡后待用。

4.1.5水浴解冻保温箱中加入清洁自来水,水深大于10cm，放入冰袋或者冰块，保持水温为2℃～

5℃。

4.2用液

4.2.1按照附录B配制所有用液。

4.2.2所有用液使用前置于2℃～5℃操作保温箱中，温度平衡后待用。

4.2.3所有用液与精液吸取前应摇匀。

1

DB43/T2412—2022

4.3公鸡挑选与训练

按NY/T3458规定执行。

4.4操作环境

4.4.1操作环境应避免污染。

4.4.2冷冻精液制作室环境温度为