DB51/T 2906-2022 猪盖塔病毒分离与鉴定技术规范

ICS11.220

CCSB41

DB51

四川省地方标准

DB51/T2906—2022

猪盖塔病毒分离与鉴定技术规范

Technicalspecificationforisolationandidentificationofswinegaetavirus

2022-05-20发布2022-07-01实施

四川省市场监督管理局发布

DB51/T2906—2022

目次

前言.................................................................................II

1范围...............................................................................1

2规范性引用文件.....................................................................1

3术语和定义.........................................................................1

4缩略语.............................................................................1

5试剂与设备.........................................................................1

5.1主要试剂.......................................................................1

5.2主要仪器设备...................................................................2

6样品的采集、保存与运输.............................................................2

6.1猪只临床发病特征...............................................................2

6.2临床样品的采集.................................................................2

6.3保存与运输.....................................................................2

7病毒的细胞分离培养.................................................................2

7.1生物安全措施...................................................................2

7.2样品的处理.....................................................................2

7.3单层细胞制备...................................................................2

7.4接种细胞.......................................................................2

7.5病毒收获.......................................................................3

8病毒核酸RT-PCR鉴定................................................................3

8.1核酸提取和反转录...............................................................3

8.2RT-PCR扩增.....................................................................3

9结果判定...........................................................................4

9.1RT-PCR.........................................................................4

9.2病毒分离鉴定...................................................................4

附录A（规范性）试剂配制.............................................................5

附录B（资料性）猪源盖塔病毒CAP基因扩增序列.........................................6

I

DB51/T2906—2022

前言

本文件按照GB/T1.1—2020《标准化工作导则第1部分：标准化文件的结构和起草规则》的规定

起草。

本文件由四川省农业农村